Scientists&Research
Xiangshu Jin, Ph.D.
- Information
- Education
- Experience
- Research
- Publication
Xiangshu Jin, Ph.D.
Assistant Investigator, NIBS, Beijing，China
Phone：86-10-80726688
Fax： 86-10-80726689
E-mail：jinxiangshu@nibs.ac.cn
Education
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2002 |
Ph. D., Chemistry, Michigan State University, East Lansing, MI, USA
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1995 |
B.S., Chemistry, Peking University, Beijing, China
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Professional Experience
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Assistant Investigator, National Institute of Biological Sciences, Beijing, China |
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Research Specialist, Howard Hughes Medical Institute; Adjunct Associate Research Scientist, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA |
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Associate Research Scientist, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA |
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Postdoctoral Research Fellow, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA |
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Postdoctoral Research Scientist, Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI, USA |
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Assistant, Conservation Laboratory, School of Archaeology and Museology, Peking University, Beijing, China |
Research Description
Our laboratory is interested in understanding the molecular “languages” of cell-cell communication that coordinates biological pattern formation. Formation of multicellular patterns associated with specific biological functions entails intricate communication networks whereby cells talk to each other through specific interactions between surface receptors and ligands presented from neighboring cells. These extracellular molecular cues are then transduced into appropriate intracellular responses, which allow a group of cells to coordinate their activities and collectively form elaborate multicellular patterns. We use a multidisciplinary approach combining structural biology, biophysics, biochemistry, cell biology, and computational biology to study the mechanisms of biological pattern formation at the atomic, molecular, cellular, and systems levels.
Our current efforts are focused on understanding the molecular interactions that underlie the establishment of planar polarity, a form of multicellular organization that coordinates polarization of cells within a tissue plane and iscritical for diverse developmental processes. To this end, we take a multipronged approach to study the extracellular interactions mediated by the CELSR family adhesion GPCRs and related adhesion receptors, the activation mechanisms of these receptors, and their downstream signaling pathways.Publications
Peer-reviewed research articles:
1. Cao Y*, Pan Y*, Huang H*, Jin X, Levin EJ, Kloss B, Zhou M (2013) Gating of the TrkH ion channel by its associated RCK protein TrkA. Nature 496, 317-322.(*equal contribution)
2. Harrison OJ, Vendome J, Brasch J, Jin X, Hong S, Katsamba PS, Ahlsen G, Troyanovsky RB, Troyanovsky SM, Honig B, Shapiro L (2012) Nectin ectodomain structures reveal a canonical adhesive interface. Nature Structural and Molecular Biology 906-915.
3. Glaaser IW, Osteen JD, Puckerin A, Sampson KJ, Jin X, Kass RS (2012)Perturbation of sodium channel structure by an inherited long QT Syndrome mutation. Nature Communications 3, 706.
4. Jin X*, Walker MA*, Felsovalyi K*, Vendome J*, Bahna F, Mannepalli S, Cosmanescu F, Ahlsen G, Honig B, Shaprio L (2012) Crystal structures ofDrosophila N-cadherin ectodomain regions reveal a widely used class of Ca2+-free interdomain linkers. Proc. Natl. Acad. Sci. U.S.A. 109, E127-134. (*equal contribution)
5. Vendome J*, Posy S*, Jin X, Bahna F, Ahlsen G, Shapiro L, Honig B (2011)Molecular design principles underlying β-strand swapping in the adhesive dimerization of cadherins. Nature Structural and Molecular Biology 18, 693-700. (*equal contribution)
6. Cao Y, Jin X*, Levin EJ*, Huang H*, Zong Y*, Quick M, Weng J, Pan Y, Love J, Punta M, Rost B, Hendrickson WA, Javitch JA, Zhou M (2011) Crystal structure of a phosphorylation-coupled saccharide transporter. Nature 473, 50-54.(*equal contribution)
7. Cao Y*, Jin X*, Huang H*, Derebe MG, Levin EJ, Kabaleeswaran V, Pan Y, Punta M, Love J, Weng J, Quick M, Ye S, Kloss B, Bruni R, Martinez-Hackert E, Hendrickson WA, Rost B, Javitch JA, Rajashankar KR, Jiang Y, Zhou M (2011) Crystal structure of a potassium ion transporter TrkH. Nature 471, 336-340. (*equal contribution)
8. Harrison OJ*, Jin X*, Hong S, Bahna F, Ahlsen G, Brasch J, Wu Y, Vendome J, Felsovalyi K, Hampton CM, Troyanovsky RB, Ben-Shaul A, Frank J, Troyanovsky SM, Shapiro L, Honig B (2011) The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins. Structure 19, 244-256. (*equal contribution)
9. Wu Y, Jin X, Harrison O, Shapiro L, Honig BH, Ben-Shaul A. (2010) Cooperativity between trans and cis interactions in cadherin-mediated junction formation. Proc. Natl. Acad. Sci. U.S.A. 107, 17592-17597.
10.Koehnke J*, Katsamba PS*, Ahlsen G, Bahna F, Vendome J, Honig B, Shapiro L,Jin X* (2010) Splice form dependence of beta-neurexin/neuroligin binding interactions. Neuron 67, 61-74. (*equal contribution)
11.Harrison OJ, Bahna F, Katsamba PS, Jin X, Brasch J, Vendome J, Ahlsen G, Carroll KJ, Price SR, Honig B, Shapiro L (2010) Two-step adhesive binding by classical cadherins. Nature Structural and Molecular Biology 17, 348-357.
12.Ciatto C, Bahna F, Zampieri N, VanSteenhouse HC, Katsamba PS, Ahlsen G, Harrison OJ, Brasch J, Jin X, Posy S, Vendome J, Ranscht B, Jessell TM, Honig B, Shapiro L (2010) T-cadherin structures reveal a novel adhesive binding mechanism. Nature Structural and Molecular Biology 17, 339-347.
13.Koehnke J, Jin X, Trbovic N, Katsamba PS, Brasch J, Ahlsen G, Scheiffele P, Honig B, Palmer AG 3rd, Shapiro L (2008) Crystal structures of β-neurexin 1 and β-neurexin 2 ectodomains and dynamics of splice insertion sequence 4.Structure 16, 410-421.
14.Koehnke J*, Jin X*, Budreck EC, Posy S, Scheiffele P, Honig B, Shapiro L(2008) Crystal structure of the extracellular cholinesterase-like domain from neuroligin-2. Proc. Natl. Acad. Sci. U.S.A. 105, 1873-1878. (*equal contribution)
15.Jin X, Townley R, Shapiro L (2007) Structural insight into AMPK regulation: ADP comes into play. Structure 15, 1285-1295.
16.Lishko PV*, Procko E*, Jin X*, Phelps CB, Gaudet R (2007) The ankyrin repeats of TRPV1 bind multiple ligands and modulate channel sensitivity. Neuron 54, 905-918. (*equal contribution)
17.Jin X, Touhey J, Gaudet R (2006) Structure of the N-terminal ankyrin repeat domain of the TRPV2 ion channel. J. Biol. Chem. 281, 25006-25010.
18.Bejar CM, Jin X, Ballicora MA, Preiss J (2006) Molecular architecture of the glucose 1-phosphate site in ADP-glucose pyrophosphorylases. J. Biol. Chem.281, 40473-40484.
19.Jin X, Ballicora MA, Preiss J, Geiger JH (2005) Crystal structure of potato tuber ADP-glucose pyrophosphorylase. EMBO J. 24, 694-704.
20.Jin X, Foley KM, Geiger JH (2004) The structure of the
21.Jin X, Geiger JH (2003) Structures of NAD+-and NADH-bound 1-L-myo-inositol 1-phosphate synthase. Acta. Crystallogr. Sect. D: Biol. Crystallogr.59, 1154-1164.
Invited reviews and book chapters:
1. Rohs R*, Jin X*, West SM, Joshi R, Honig B, Mann RS (2010) Origins of specificity in protein-DNA recognition. Annu. Rev. Biochem. 79, 233-269. (*co-first authors)
2. Geiger JH, Jin X (2006) The structure and mechanism of myo-inositol-1-phosphate synthase. Subcell. Biochem. 39, 157-180.
