李 祺 博士
遗传筛选中心主任
电话：010-80726688-8677
Email: liqi@nibs.ac.cn

教育经历
2004-2008年 山东师范大学生命科学学院，生物技术学士学位
2008-2016年 北京大学生命科学学院，生理学博士学位

工作经历
2016-2017年 北京大学生命科学学院，博士后
2017-2021年 北京生命科学研究所，博士后

中心职能

遗传筛选中心Genetic Screening Center:

腺相关病毒是目前应用最广泛的基因递送工具之一，具有低免疫原性，低整合性，高侵染性，及高稳定性持续表达等优点，被广泛应用于基础科学研究和临床基因治疗。北京生命科学研究所遗传筛选中心为研究所各实验室提供使用咨询，克隆构建，包装纯化，滴度测定，效果测试等与病毒载体相关的技术服务与支撑。中心目前可稳定包装生产多种血清型的腺相关病毒载体。同时，中心也会根据各实验室的需求，开发新型的腺相关病毒载体，以高效特异侵染感兴趣的组织细胞，给各实验室的研究提供助力。
Adeno-associated virus (AAV) is currently one of the most widely used gene delivery tools in both scientific research and gene therapy. AAV has many advantages, including low immunogenicity, low genomic integration, high transduction rate, and stable and persistent expression. The Genetic Screening Center provides AAV technical services including vector construction, packaging, purification, titration and functional test for different labs. The Genetic Screening Center provides multiple common serotypes of recombinant Adeno-associated virus and helps construct AAVs for either expression or knockout of target genes. Moreover, the center is committed to develop and evolve new serotype vectors according to different labs’ demand and interest.

发表文章

1. Cao, Y., J. Zheng, H. Wan, Y. Sun, S. Fu, S. Liu, B. He, G. Cai, Y. Cao, H. Huang, Q. Li, Y. Ma, S. Chen, F. Wang and H. Jiang (2023). "A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease." EMBO J 42(13): e113033.

2. He, B., H. Yu, S. Liu, H. Wan, S. Fu, S. Liu, J. Yang, Z. Zhang, H. Huang, Q. Li, F. Wang, Z. Jiang, Q. Liu and H. Jiang (2022). "Mitochondrial cristae architecture protects against mtDNA release and inflammation." Cell Rep 41(10): 111774.

3. Wang, G.*, Q. Li*, J. Xu, S. Zhao, R. Zhou, Z. Chen, W. Jiang, X. Gao, S. Zhou, Z. Chen, Q. Sun, C. Ma, L. Chen, B. Shi, Y. Guo, H. Wang, X. Wang, H. Li, T. Cai, Y. Wang, Z. Chen, F. Wang and Q. Liu (2022). "Somatic genetics analysis of sleep in adult mice." J Neurosci 42(28): 5617-5640.

4. Zhou, R.*, G. Wang*, Q. Li, F. Meng, C. Liu, R. Gan, D. Ju, M. Liao, J. Xu, D. Sang, X. Gao, S. Zhou, K. Wu, Q. Sun, Y. Guo, C. Wu, Z. Chen, L. Chen, B. Shi, H. Wang, X. Wang, H. Li, T. Cai, B. Li, F. Wang, H. Funato, M. Yanagisawa, E. E. Zhang and Q. Liu (2022). "A signalling pathway for transcriptional regulation of sleep amount in mice." Nature 612(7940): 519-527.

5. Deng, B.*, Q. Li*, X. Liu*, Y. Cao*, B. Li, Y. Qian, R. Xu, R. Mao, E. Zhou, W. Zhang, J. Huang and Y. Rao (2019). "Chemoconnectomics: Mapping Chemical Transmission in Drosophila." Neuron 101(5): 876-893 e874.

6. Petruccelli, E., Q. Li, Y. Rao and T. Kitamoto (2016). "The Unique Dopamine/Ecdysteroid Receptor Modulates Ethanol-Induced Sedation in Drosophila." J Neurosci 36(16): 4647-4657.

7. Song, S., Y. Yuan, J. Lu, Q. Li, Z. Zhu, Q. Fan, Y. Xue, Z. C. Lai and W. Zhang (2013). "The Drosophila ortholog of breast cancer metastasis suppressor gene, dBrms1, is critical for developmental timing through regulating ecdysone signaling." Dev Biol 380(2): 344-350.