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实验室

13:30-15:30, Wednesday, December 26, 2018


Speaker1: Peng Zou, Ph.D.

College of Chemistry and Molecular Engineering

Peking University

Topic: Beyond fluorescent proteins: hybrid voltage indicators for imaging bioelectricity

Abstract

Optical biosensors have been invaluable tools in neuroscience research, as they provide the ability to directly visualize neural activity in real time and with exceptional spatial and temporal resolutions. Notably, a majority of these sensors are based on fluorescent proteins, which offer the ability to target specific cell types or even subcellular compartments. However, fluorescent proteins are intrinsically bulky tags whose photophysical properties are often inferior to synthetic dyes. In this talk, I will present a hybrid fluorescent voltage indicator that combines the advantages of genetically-encoded protein scaffold and small-molecule fluorophore. Our method is built upon the site-specific modification of microbial rhodopsin proteins with organic fluorophores, resulting in a novel indicator structure that represents one of the most sensitive and fastest orange-colored voltage indicators developed to date. We applied this technique to optically map electrical connectivity in cultured cells, which revealed gap junction-mediated long-range coupling that spanned over hundreds of micrometers.

Speaker2: Louis Tao, Ph.D.

Center for Bioinformatics

School of Life Sciences

Peking University

Topic: Using Optical Imaging to Infer Function and Map Connectivities in Neuronal Circuits

Abstract

The ability to map functional connectivity is necessary for the study of the flow of activity and its function in neuronal circuits. Optical imaging of calcium indicators is an important

adjunct to electrophysiology and is widely used to visualize neuronal activity. Here we present two examples of mapping neural circuits from optical imaging data. In the first, we examine larval zebrafish central nervous system undergoing chemoconvulsant induced seizures. Our automatic functional connectivity mapping procedure provides a practical and user-independent means for summarizing the flow of activity through neural pathways. In the second, by using our own imaging setup that allows us to record simultaneously worm behavior and motor neurons, we can begin to study the neural circuit underlying worm locomotion.

Host:  Meng-Qiu Dong, Ph.D.