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实验室

11:00-12:00, Wednesday, May 2, 2018


Speaker: Liang Gao., Ph.D.

Research Scientist and Project Lead

Intelligent Imaging Innovations, Inc., Denver, CO, 80216

Topic: Pushing the envelop in 3D fluorescence imaging

Host: Minmin Luo, Ph.D.

Abstract

Despite the progress made in 3D fluorescence imaging techniques, high-resolution, high-speed 3D live imaging of large multicellular specimens is still challenging.  Tiling light sheet selective plane illumination microscopy (TLSSPIM) with real-time light sheet optimization ability was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes its imaging performance by using a realtime adjustable tiling light sheet.

In TLS-SPIM, a small but thin light sheet is tiled quickly within the image plane to illuminate. The entire FOV, while only the fluorescence signal generated at center of each tiled light sheet Is used in the final image. As a result, high spatial resolution and good optical sectioning capability are obtained in a FOV that is much larger than the light sheet itself. In addition, TLS-SPIM enables the implementation of the latest SPIM light sheets to optimize its imaging ability in real-time.