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实验室

12:30-13:30, Tuesday, March 20, 2018


Speaker: Aibin He(何爱彬), Ph.D.

Assistant Professor

Center for Life Sciences,

Peking University Institute of Molecular Medicine

Topic: Single-cell transcriptomics reveals lineage hierarchies and interlineage communications for early heart development

Host: Nan Tang , Ph.D.

Abstract

何爱彬实验室致力于研究心脏发育起源与再生的细胞与分子机制。我们的研究集中在如下两个方向:1. 胚胎期心脏干细胞特化与分化的表观遗传调控机制。我们利用/发展单细胞转录组与表观遗传学技术等手段,探索心脏干细胞特化与命运决定的关键增强子组与其调控的转录因子网络。2. 实时成像结合数学模型绘制心脏发育起源/心脏再生的细胞学图谱。 我们利用光片显微镜与图像图形处理技术,结合细胞谱系示踪,追踪心脏干细胞的增殖、分化、迁移和分配,绘制细胞图谱。本实验室综合运用发育生物学、表观遗传学、生物信息学、光片显微镜技术和数学建模等多学科交叉工具与方法,解决上述科学问题。

Research Area:

Fascinated by precisely controlled cardiac progenitor cells (CPCs) differentiation and allocation during heart development, we mainly focus on understanding of cellular origins and fates, and epigenetic mechanisms of cardiogenesis and heart regeneration. Particularly, our research directions include but are not limited to the following two aims: Aim 1 is to probe epigenetic underpinnings of CPCs specification and fate choice determination at single-cell level. To this end, we will combine single-cell transcriptomic profiling with in-house developed new single-cell epigenomic techniques to define the key intermediate lineages specific enhancers and master transcription factors centered regulatory network. Aim 2 is to construct digital cell lineage roadmap of early heart development and regeneration through custom digital scanned light-sheet microscopy. The goal of this aim is to map heart cells’ behaviors, including proliferation, specification, and allocation during differentiation to form distinct parts of the heart. The multi-disciplinary knowledge from developmental biology, epigenetics, bioinformatics, optical microscopy and mathematical modeling will be harnessed to tackle these above questions.