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Meng-Qiu Dong

北京生命科学研究所研究员,化学中心主任
Meng-Qiu Dong, Ph.D.
Assistant Investigator, NIBS, Beijing, China
Phone: 010-80726688-8515
Fax: 010-8072-6689
E-mail: dongmengqiu@nibs.ac.cn

Education

2001 Ph.D., Department of Molecular Biophysics & Biochemistry, Yale University, USA
1995 M.Sc., Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China
1992 B.Sc., Department of Biology, Sichuan University, China

Professional Experience

2007- Assistant Investigator, National Institute of Biological Sciences, Beijing, China
2003-2007 Postdoctoral Research Associate, The Scripps Research Institute, USA
2001-2003 Postdoctoral researcher, UCSD School of Medicine, USA

Research Description

Our lab is a place where biology intertwines with mass spectrometry.

On the biology side, our lab is interested in understanding the regulatory mechanisms of aging using C. elegans as a model. We quantify mRNA and protein abundance changes during the aging process of wild type and long-lived C. elegans mutants, and ask how they differ. We also analyze what happens to sub-cellular organelles such as mitochondria during aging. By cataloging such changes, we hope to describe the aging process in more detail, and lay the foundation for further investigations about the regulation of aging. At the center stage of our aging research is the Insulin/IGF-1 Signaling (IIS) pathway. Although it has been shown that components of the IIS pathway, from the insulin receptors to the FoxO transcription factors, all have important functions in lifespan regulation, the exact target genes downstream of FoxO that are responsible for the longevity of IIS mutants remain unclear. We are particularly interested in finding out those targets and then discerning the connections amongst them so that one can begin to understand the extraordinary effect of IIS on lifespan. We are also actively studying post-translational modifications (PTM) of DAF-16, which is the C. elegans FoxO. This key transcription factor is intricately regulated by a battery of kinases and a couple of phosphatases, including calcineurin, a calcium-and-calmodulin-dependent Ser/Thr phosphastase. The questions we are trying to address include how different environmental and internal cues (e.g. developmental stages) affect PTMs of DAF-16, and how the "modification code" of DAF-16 alters its transcriptional activity.

On the mass spectrometry (MS) front, our goal is to use and develop MS-based proteomic techniques to help answer biological questions. Besides protein identification, we routinely carry out quantitative proteomics analysis and PTM analysis of samples as complex as cell lysates. In collaboration with other researchers including the pFind group at the Institute of Computing Technology, we are currently developing methods to sequence peptides de novo (i.e. without searching a protein sequence database), to improve peptide identification from ETD spectra, and to probe protein-protein interactions by crosslinking followed by MS identification. The lab is equipped with four mass spectrometers, a Q Exactive, an LTQ-orbitrap with ETD, an ion trap instrument HCTultraPTM, and a triple-quadruple instrument TSQ Discovery.

Publications

  1. Coffman K, Yang B, Lu J, Tetlow AL, Pelliccio E, Lu S, Guo DC, Tang C, Dong MQ*, Tamanoi F* (2014) Characterization of the Raptor/4E-BP1 interaction by chemical cross-linking coupled with mass spectrometry analysis. J Biol Chem. 289(8): 4723-34

  2. Shen EZ, Song CQ, Lin Y, Zhang WH, Su PF, Liu WY, Zhang P, Xu J, Lin N, Zhan C, Wang X, Shyr Y, Cheng H*, Dong MQ* (2014) Mitoflash frequency in early adulthood predicts lifespan in Caenorhabditis elegans. Nature [published online Feb 12. 2014]

  3. Coffman K, Yang B, Lu J, Tetlow AL, Pelliccio E, Lu S, Guo DC, Tang C, Dong MQ*, Tamanoi F* (2014) Characterization of the Raptor/4E-BP1 interaction by chemical cross-linking coupled with mass spectrometry analysis. J Biol Chem. [published online Jan 8. 2014]

  4. Li TM, Chen J, Li X, Ding XJ, Wu Y, Zhao LF, Chen S, Lei X*, Dong MQ* (2013) Absolute Quantification of a Steroid Hormone that Regulates Development in Caenorhabditis elegans. Anal Chem. 85(19):9281-7

  5. Sun LL, Li M, Suo F, Liu XM, Shen EZ, Yang B, Dong MQ, He WZ, Du LL (2013) Global analysis of fission yeast mating genes reveals new autophagy factors. PLoS Genet. 9(8):e1003715

  6. Tao L, Xie Q, Ding YH, Li ST, Peng S, Zhang YP, Tan D, Yuan Z*, Dong MQ* (2013) CAMKII and Calcineurin Regulate the Lifespan of Caenorhabditis elegans through the FOXO Transcription Factor DAF-16. eLife 2:e00518

  7. Ding YH, Du YG, Luo S, Li YX, Li TM, Yoshina S, Wang X, Klage K, Mitani S, Ye K*, Dong MQ* (2013) Characterization of PUD-1 and PUD-2, Two Proteins Up-Regulated in a Long-Lived daf-2 Mutant. PLoS One 8(6):e67158

  8. Li MM, Nilsen A, Shi Y, Fusser M, Ding YH, Fu Y, Liu B, Niu Y, Wu YS, Huang CM, Olofsson M, Jin KX, Lv Y, Xu XZ, He C, Dong MQ, Rendtlew Danielsen JM, Klungland A, Yang YG. (2013) ALKBH4-dependent demethylation of actin regulates actomyosin dynamics. Nat Commun. 4:1832

  9. Zhai Q, Yan L, Tan D, Chen R, Sun J, Gao L, Dong MQ, Wang Y, Li C. (2013) Phosphorylation-coupled proteolysis of the transcription factor MYC2 is important for jasmonate-signaled plant immunity. PLoS Genet. 9(4):e1003422

  10. Chi H, Chen H, He K, Wu L, Yang B, Sun RX, Liu J, Zeng WF, Song CQ, He SM*, Dong MQ* (2013) pNovo+: De Novo Peptide Sequencing Using Complementary HCD and ETD Tandem Mass Spectra. J Proteome Res. 12(2):615-25

  11. Yang B, Wu YJ, Zhu M, Fan SB, Lin J, Zhang K, Li S, Chi H, Li YX, Chen HF, Luo SK, Ding YH, Wang LH, Hao Z, Xiu LY, Chen S, Ye K, He SM*, and Dong MQ* (2012) Identification of Cross-linked Peptides from Complex Samples. Nature Methods 9(9):904-6

  12. Qu M, Yang B, Tao L, Yates JR 3rd, Russell P, Dong MQ, Du LL (2012) Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint. PLoS Genet. 8(7):e1002817

  13. Xie Q, Hao Y, Tao L, Peng S, Rao C, Chen H, You H, and Dong MQ, Yuan Z (2012) Lysine Methylation of FOXO3 Regulates Oxidative Stress-induced Neuronal Cell Death. EMBO Reports 13(4):371-7

  14. Zhao Y, Sun W, Zhang P, Chi H, Zhang M, Song CQ, Ma X, Shang Y, Wang B, Hu Y, Hao Z, Huhmer A, Meng F, L'Hernaultf S, He SM, Dong MQ*, Miao L* (2012) Nematode Sperm Maturation Triggered by Protease Involves Sperm-secreted Serine Protease Inhibitor (Serpin). Proc Natl Acad Sci U S A. 109(5):1542-7

  15. Li S, Duan J, Li D, Yang B, Dong M, Ye K. (2011) Reconstitution and structural analysis of the yeast box H/ACA RNA-guided pseudouridine synthase. Genes Dev. 25(22):2409-21.

  16. Li J, Wang J, Hou W, Jing Z, Tian C, Han Y, Liao J, Dong MQ, Xu X. (2011) Phosphorylation of Ataxin-10 by polo-like kinase 1 is required for cytokinesis. Cell Cycle. 10(17):2946-58

  17. Zheng M, Wang YH, Wu XN, Wu SQ, Lu BJ, Dong MQ, Zhang H, Sun P, Lin SC, Guan KL, Han J. (2011) Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1. Nat Cell Biol. 13(3):263-72.

  18. Sun RX*, Dong MQ*, Song CQ, Chi H, Yang B, Xiu LY, Tao L, Jing ZY, Liu C, Wang LH, Fu Y, He SM (2010) Improved Peptide Identification for Proteomic Analysis Based on Comprehensive Characterization of Electron Transfer Dissociation Spectra. J Proteome Res. 9(12): 6354-67

  19. Li YH, Dong MQ, Guo Z. (2010) Systematic analysis and prediction of longevity genes in Caenorhabditis elegans. Mech Ageing Dev. 131(11-12):700-9.

  20. Wang SF, Kou ZH, Jing ZY, Zhang Y, Guo XZ, Dong M, Wilmut I, Gao SR (2010) Proteome of mouse oocytes at different developmental stages. Proc Natl Acad Sci U S A. 107(41): 17639-44.

  21. Chi H, Sun RX, Yang B, Song CQ, Wang LH, Liu C, Fu Y, Yuan ZF, Wang HP, He SM*, Dong MQ* (2010) pNovo: De novo Peptide Sequencing and Identification Using HCD Spectra. J Proteome Res. 9(5): 2713-24

  22. Sun RX*, Dong MQ,* Chi H, Yang B, Xiu LY, Wang LH, Fu Y, He SM (2010) ECD/ETD-based tandem mass spectrometry in proteomics. Progress in Biochemistry and Biophysics 37(1): 94-102. Review. (Chinese)

  23. Zhang DW, Shao J, Lin J, Zhang N, Lu BJ, Lin SC, Dong MQ, Han J. (2009) RIP3, an Energy Metabolism Regulator that Switches TNF-Induced Cell Death from Apoptosis to Necrosis. Science 325(5938): 332-6

Address:7 Science Park Rd., ZGC life science park, Beijing 102206 Tel:+86-10-80726688 Fax:+86-10-80726689 E-mail:info@nibs.ac.cn
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